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A workflow for whole-genome sequencing of bacterial isolates

Written by Oxford Nanopore
Company news Drug discovery and development Microbiology PCR and sequencing Whole-genome studies

Whole-genome sequencing of microbial isolates provides valuable information for public health, clinical microbiology research, food safety and microbial ecology.

Nanopore sequencing offers distinct advantages when it comes to detection and characterization of antimicrobial resistance and virulence genes, plasmids and other mobile genetic elements.

Download this rapid end-to-end workflow to learn about the nanopore-only microbial isolate sequencing solution (NO-MISS) and discover a flexible and rapid approach for whole-genome sequencing of bacterial isolates.

In this workflow, you’ll learn more about:

  • Extraction: cell lysis and obtaining high-quality DNA
  • Library preparation: sample multiplexing
  • Sequencing: achieving ultimate flexibility with MinION Flow Cells
  • Analysis: using the EPI2ME bacterial genomes workflow

This content was provided by Oxford Nanopore Technologies.

 

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