Back to basics: flow cytometry

Sonal Manohar is an Assistant Professor at the Sunandan Divatia School of Science (Mumbai, India) specializing in cancer biology and cell signaling. She has recently co-authored two papers published in Bioanalysis, one of which covers the principles, applications and recent advances in flow cytometry and another focusing on cell-cycle analysis using flow cytometry. Here, she explains the principles of flow cytometry and shares advice for those using the technique for the first time. What is flow cytometry? Flow cytometry is a technique that enables rapid analysis of a statistically significant number of cells at a single-cell level and allows for...