Scientific Article: Recombinant protein expression, challenges and solutions
High-quality recombinant proteins are important starting materials for successful research efforts and drug development campaigns. Key attributes of the quality of any recombinant protein include purity, oligomeric status, thermo and chemical stability, folding, post-translational modifications (PTMs), and activity. Due to the intrinsic complexity of proteins in general, protein expression and purification are often challenging. It usually requires a systematic optimization effort to formulate a workable procedure to express high-quality target proteins.
This Scientific Article gives an overview of recombinant protein expression, summarizes the common challenges often encountered in obtaining high-quality protein-of-interest as well as new challenges brought forward by high-throughput expression, and explores approaches to optimization by using a few case studies to demonstrate how to optimize key components in the recombinant protein expression workflow.
In this Scientific Article you will learn about:
- An overview of recombinant protein expression
- Challenges in obtaining high-quality protein-of-interest and high-throughput expression
- Approaches to optimization and case studies
And much more!
This content was provided by Sino Biological.