Functional characterization of monoclonal antibodies using kinetic live-cell analysis
This White Paper demonstrates how various biological activities of the ADC Kadcyla® can be characterized in real-time using the Incucyte® Live-Cell Analysis System. The system combines non-perturbing cell visualization and data acquisition with integrated analysis to provide kinetic insights about mechanism of action and other effects.
Antibody-drug conjugates (ADCs) are among the fastest growing class of biologics and are developed as a combination of monoclonal antibodies (mAbs) and highly potent cytotoxic molecules (drugs). The antibody, in addition to its own biological specificity through its paratope, has the function of a targeted transporting tool of the drug to the targeted cell. The conjugated molecules or particles typically have cytotoxic activity and thus increase the efficiency of the antibody by an additional mechanism, leading to increased death of cells carrying the target antigen.
Conjugation of these drugs to antibodies, however, increases structural complexity, which triggers the need for improved characterization methods utilizing technologies that can add biophysical and functional information.
In this study we explore:
- The characterization of Kadcyla® in HER2 positive monocultures
- The effects of Kadcyla® in an antibody-dependent cellular cytotoxicity co-culture model
- How to leverage real-time kinetic data in antibody discovery workflows
This content was provided by Sartorius