Application Note: Label-free, real-time live-cell assays for 3D organoids embedded in Matrigel®
This application note describes the use of the Incucyte® Live-Cell Analysis System along with the Incucyte® Organoid Analysis Software Module to study the growth or death of organoids, label-free. A proprietary brightfield image acquisition approach enables real-time kinetic imaging of 3D organoids embedded within a matrix (Matrigel®). Organoid size, count and morphology measurements are automatically plotted over time to gain in-depth organoid characterization following perturbation.
Here, Sartorius (Gottingen, Germany) describes validation methods and data demonstrating the ability to kinetically image and quantify the growth, death and morphology of organoids embedded in Matrigel®.
Advances in preclinical in vitro models are crucial for both basic research and drug development across a range of applications. Organoid technologies are increasingly being used as in vitro models of human development and disease as they exhibit structural, morphogenetic and functional properties that recapitulate in vivo pathophysiology. To successfully use these models across a variety of research disciplines and applications, approaches that reduce variability and technology pipelines to image and quantify these complex cell models are required.
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