Application Note: Quantifying T-cell response in 3D tumor spheroids using advanced flow-cytometry workflows

The discovery of novel immunotherapies that specifically target and enhance the T-cell response against cancer, for example CAR-T cells, bispecific antibodies and checkpoint inhibitors, is a rapidly expanding research area. Robust and relevant in vitro models for the evaluation of these immunotherapies are essential throughout their development. Recent evidence supports the use of 3D advanced tumor models, such as multicellular spheroids or organoid cultures, as opposed to the 2D monolayer. However, traditional methods for measuring T-cell response in 3D models, such as imaging-based techniques, are often limited. In this Application Note, Sartorius provides two advanced flow-cytometry-based solutions to address these...