FFPE-derived DNA: top tips for constructing a next-generation sequencing library

What role does formalin-fixed paraffin embedding (FFPE) play in clinical and translational research? Not only a common method of preservation, FFPE also allows researchers to study tissue samples, potentially contributing to future clinical applications. However, to ensure successful and reliable sequencing of FFPE-derived DNA, it is necessary to conduct quality control (QC) of the initial DNA and fragmented samples at key points during next-generation sequencing (NGS) library construction. This Application Note describes how a high-quality whole-exome sequencing library can be obtained by considering QC steps throughout the library preparation process, including the initial sample, pre- and post-PCR steps. You can...