Organoid analysis guide
The starting point for developing of organoid cultures can be tissue-specific, resident adult stem cells, cancer stem cells derived from patient biopsies, or pluripotent stem cells, either embryonic or induced. When established with 3D extracellular matrices, the cultures can recapitulate the in vivo architecture, spatial organization, and genetic diversity of the cell populations found in the original organ with remarkable fidelity. As self-organizing and self-renewing 3D structures, organoids offer a distinct advantage over traditional monolayer culture techniques and offer a more physiologically relevant milieu in which to understand complex biology with greater clarity.
In this guide, we explore some of the translational applications of organoids, highlight key considerations for successful 3D culture and expansion in vitro and describe leading-edge technology for the imaging and objective analysis of these complex, self-organizing tissues.
In this guide, find more information about:
- Translational applications
- Organoid culture and analysis
- Real-time live-cell analysis
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