Application Note: Best practices for pipetting 3D cell culture hydrogels

Poor pipetting technique causes low reproducibility between samples and experiments. Preparing 3D cell cultures is challenging and prone to variability due to the hydrogels, pipettes and tips used in the process, but most importantly due to individual pipetting practices. With electronic pipettes, you can use protocols with fixed pipetting speeds and mixing steps, consequently minimizing the variability introduced by individual pipetting practices. In this Application Note, Sartorius show how you can achieve reproducible 3D cell culture results with electronic Picus® Nxt pipettes and GrowDex® hydrogel. This content was provided by Follow Sartorius on Twitter, Facebook and on LinkedIn.