Featured journal article: Bringing the tumor microenvironment into focus
Multiplex immunohistochemistry-immunofluorescence (mIF) panel development enables simultaneous detection of multiple proteins of interest within a single sample, advantageous in analysis of limited tissue such as tumor biopsy. The applications of mIF span clinical and basic research, but a 7-color mIF can take ≥8 weeks to develop.
Here, a faster, simplified approach to 7-color mIF panel development is described with a focus on the tumor microenvironment.