Single-cell sequencing

The advent of single-cell sequencing has revolutionized the genomics and transcriptomics space, allowing researchers to delve deeper into cell properties like gene expression than previous methods, which simply provided ensemble average measurements of all the cells in a sample. The technique has has such an impact that it has been recognized as Nature’s Method of the Year twice in one decade; it was first awarded for its initial development in 2013 and then for the addition of multimodal omic capabilities in 2019.

Despite the meteoric rise of this technique, significant challenges remain: vast amounts of data are produced by these studies, which can be challenging to analyze and interpret, and there are key aspects to consider in sample preparation.

In this In Focus, supported by 10x Genomics, we will explore:

• Exciting new applications of single-cell methods
• Key considerations for sample preparation
• Overcoming challenges in data analysis