A peek behind the paper: A comprehensive guide to dynamic analysis of microbial gene expression using the 3D-printed PFIbox and a fluorescent reporter library

Discover a new method, providing fluorescence imaging under restrictive incubation settings at a high resolution, with parallel imaging capabilities. Here, authors Shawn French, Amelia Guo and Eric Brown take you through their recent paper guiding readers to create their own modified and optimized Printed Fluorescence Imaging boxes (PFIboxes). Please provide a short summary of your paper “A comprehensive guide to dynamic analysis of microbial gene expression using the 3D-printed PFIbox and a fluorescent reporter library,” published in Nature Protocols. In our manuscript, we illustrate step-by-step how to create, assemble, and program PFIboxes. This is a complicated process that involves several...