Considerations for building an accurate shotgun metagenomic workflow for gut microbiome profiling

The human gut microbiome plays an important role in human health and various diseases. Interest in using high-throughput DNA sequencing techniques to study the human gut microbiome has grown immensely across many sectors, from academia to industry to government. Additionally, as sequencing costs continue to drop, more researchers are considering using a shotgun metagenomic workflow rather than a targeted sequencing approach, to obtain much more information from a sample.

This article discusses important considerations for building a shotgun metagenomic workflow that can achieve accurate and reproducible microbiome profiling through real-world examples. Considerations include sample preservation, controls, DNA extraction, library preparation, sequencers, and bioinformatics. Click here to check out the ZymoBIOMICS portfolio.

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