Liquid-electron microscopy: making waves in structural biology

Liquid-electron microscopy (EM) is an established advanced characterization method, although it has only recently been making waves in the field of structural biology. In this article, Samantha Berry, Liza-Anastasia DiCecco and Deborah Kelly from the Kelly Lab at Pennsylvania State University (PSU; PA, USA), and Jennifer Gray, an Associate Research Professor at the Material Research Institute of PSU, share their advice for novel users of liquid-EM, discussing sample preparation and imaging conditions. Contents: Introduction Current methods Commercial holders Thin film liquid enclosures Hybrid microchip assemblies Sample preparation Size (and concentration) matters Imaging conditions Soft sample requirements The Goldilocks principle Conclusion Introduction In...