Peek behind the paper: In vitro amplification of whole large plasmids via transposon-mediated oriC insertion

Explore the motivations and reasoning behind a recent Benchmark paper that establishes a new method to provide a powerful tool to amplify various environmental circular DNAs. With a research background in molecular biology, particularly bacterial chromosomal replication, Masayuki Su’estugu (Rikkyo University, Tokyo, Japan) has recently succeeded in developing an in vitro reconstitution of the replication cycle reaction (RCR) for the Escherichia coli chromosome. His current research focuses on the development of new applications that use the RCR system. Here Su’estugu details his latest paper published in BioTechniques.  Please provide a short summary of your paper. RCR is a novel DNA amplification technology...