Key considerations in qPCR assay controls
Quantitative PCR (qPCR) is a widely utilized technique for the semiquantitative study of gene expression. However, its accuracy and reproducibility depend significantly on the proper use of assay controls.
This Article delves into the distinct types of assay controls crucial to ensuring reliable qPCR results.
Download for user guidance on the design and application of controls, including DNA contamination control, positive PCR control, RNA quality assessment, reverse transcription control, and the selection and validation of reference genes with the use of prevalidated assays.
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What you’ll learn:
- Control mastery: harness the critical role of controls in qPCR, mastering how to detect DNA contamination, select positive PCR controls, evaluate RNA quality, and utilize reverse transcription controls
- Selection and validation of reference genes: learn efficient strategies for identifying appropriate reference genes across diverse experimental conditions
- Introduction to multiplex PCR: explore the benefits of multiplex PCR, including increased throughput, cost-effectiveness, and heightened accuracy
- Primer design and optimization: uncover approaches for primer design and optimization, and learn validation techniques for robust multiplex PCR assays