Peek behind the paper: Optimized RNA isolation of FFPE uterine scar tissues for RNA expression analyses delineated by laser microdissection

Alexander Paping is a research associate at the Charité Universitätsmedizin Berlin (Germany) in the Division of ‘Experimental Obstetrics’ as well as a practicing obstetrician in the Department of Obstetrics. Here, he writes about his recent publication outlining a protocol for maximized RNA output from uterine wall samples. Often, samples for histological analysis are formalin-fixed paraffin-embedded (FFPE) and slide mounted, which introduces challenges to RNA extraction. In this ‘Peek behind the paper’, Paping discusses an optimized method for tissue analysis, which allows for the identification and analysis of gene expression in human maternal uterine scar tissue from uterometry scars and gives...