Peek Behind the Paper: Optimization of phosphate limited autoinduction broth for 2-Stage heterologous protein expression in E. coli

Working to improve protein expression and allow researchers to focus on the real crux of their studies, Dr. Romel Menacho-Melgar, Postdoctoral Fellow at Duke University and CSO of Roke Biotechnologies (both NC, USA), established a method to optimize protein expression at the end of his PhD. Identifying the need to simplify the product, Menacho-Melgar improved his process further.  Here, Menacho-Melgar presents an update to one of the essential components of this process: the autoinduction broth. Read on to see how this update simplifies protein expression and to get key tips for best practices and the future of protein expression. Please...