Emulsion PCR made easy
Method Summary
Here we describe a simplified method for direct breakage of the emulsion using the DNA binding buffer (containing guanidine hydrochloride and isopropanol) that is provided with commonly used silica column-based purification kits, followed by purification of the PCR products using silica columns. This method eliminates the routinely performed multi-step breakage of emulsion and extraction of DNA using hazardous organic solvents, making it simpler and safe for use in any laboratory.
Authors: Verma V, Gupta A, & Chaudhary V.