Reduced serum methods for contact-based coculture of human dermal fibroblasts and epidermal keratinocytes
Method Summary
Two reduced serum-based methods (1–2% fetal bovine serum) were formulated using commercially available media components. Human dermal fibroblasts and epidermal keratinocytes were cocultured by layering keratinocytes over confluent fibroblasts or by seeding the two cell types simultaneously. The coculture platform was leveraged to study cell migration and wound closure using scratch assays.
Authors: Kadam S, Vandana M & Kaushik K.
View the full protocol on the co-culture of fibroblasts and keratinocytes here