RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors
Method Summary
Crudely macerate plant tissue in water for a few minutes and transfer supernatant in a recombinase polymerase amplification (RPA) reaction. Incubate RPA reaction for 20 min at 39°C and thereafter directly utilize RPA reaction to exponentially amplify target through standard PCR.
Authors: Munawar M, Martin F, Toljamo A, Kokko H & Oksanen E.