Tumour cell invasion into Matrigel: optimized protocol for RNA extraction
Method Summary
An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in combination with a specific commercial kit used to perform the DNAse treatment and optimal RNA purification on column. This method ensures optimal yield, good purity and integrity for analysis of high and low molecular weight RNA molecules.
Authors: Ferretti R, Baldassarre A, de Billy E et al.