Investigating essential biomolecular pathways with cutting-edge techniques: time-resolved single-molecule biophysics methods in action
Explore the use of time-resolved fluorescence microscopy and spectroscopy to monitor key biomolecular interactions and dynamics.
This webinar will detail the use of time-resolved fluorescence methods to investigate biomolecules in action and answer fundamental biological questions. Sharonda will explain how her lab uses a time-resolved confocal fluorescence microscope to detect and image single molecules. The webinar then explores fluorescence correlation spectroscopy (FCS) and fluorescence resonance energy transfer (FRET) analysis to monitor biomolecule conformational changes, transient protein-protein or protein-nucleic acid interactions. These critical details for developing complete molecular models are only detectable at the single-molecule level.
Learn how to optimally conduct:
- Time-resolved fluorescence
- Fluorescence correlation spectroscopy (FCS)
- Fluorescence resonance energy transfer (FRET)
- Fluorescence lifetime imaging microscopy (FLIM)
Who this may interest?
- Imaging scientists and microscopists
- Spectroscopists
- Biophysicists
- Enzymologists
- Basic researchers interested in single-molecule analysis
Speaker
Sharonda Johnson LeBlanc, PhD
Assistant Professor of Physics
North Carolina State University (NC, USA)
Sharonda LeBlanc, PhD is an Assistant Professor of Physics at North Carolina State University. She graduated from the Nanoscale Science PhD program at UNC Charlotte in 2012. Her lab designs single-molecule fluorescence-based assays to investigate biomolecules in action and answer fundamental biological questions. Time-resolved fluorescence methods employed in her lab include fluorescence correlation spectroscopy (FCS) and fluorescence resonance energy transfer (FRET) to monitor fast biomolecular interactions that drive biological pathways. Her lab is primarily focused on questions related to three distinct systems: 1) SARS-CoV-2 viral RNA processing 2) ribosome assembly, and 3) DNA repair. She enjoys spending her spare time with her husband and two daughters.
This webinar was recorded on the 29 February 2024