Decoding the epigenome with Oxford Nanopore real-time methylation detection
In this webinar, we will focus on the relevance of modifications, particularly DNA methylation at the fifth position of cytosine (5mC), in studying disease mechanisms in humans. We will present benchmarking results and cover essential information for modification analysis, including modkit’s new features. Lastly, we will showcase the benefits of using native Oxford Nanopore sequencing for modification, calling in real-world applications with high-accuracy reads.
What will you learn?
- How nanopore sequencing enables accurate, real-time detection of modifications in DNA and RNA.
- About our advanced basecaller, Dorado, which accurately calls bases and modifications simultaneously.
- How to use modkit – a tool for processing modification BAM files and generating summary statistics.
- How Nanopore’s streamlined analysis workflow answers key questions in epigenetics and broader biological inquiries.
Who this may interest?
- Academic researchers
- Drug development researchers
- Clinical researchers
- Sequencing researchers
- Genomic researchers
Speakers
Rocio Esteban
Application Support Bioinformatician
Oxford Nanopore (Oxford, UK)
Rocio completed a biotechnology degree at the University of Lleida and a master’s degree in computational sciences at the University of Vigo (both Spain). Prior to joining Oxford Nanopore, she worked as a Bioinformatics Specialist for a genomics company, helping to develop various bioinformatics pipelines including genotyping projects for population genomics studies, genome assembly and annotation. She joined Oxford Nanopore 4 years ago, starting in the benchmarking team, where her work focused on methylation calling as well as germline and somatic SV calling general benchmarking. Rocio also worked on adaptive sampling, helping to develop the Reduced-Representation Methylation Sequencing (RRMS) method. Currently, she provides bioinformatic support in customer-facing projects covering a range of topics such as trio sequencing and pharmacogenomics.
Arthur Rand
Research Scientist – Machine Learning
Oxford Nanopore (Oxford, UK)
Art was introduced to nanopore sequencing and Oxford Nanopore Technologies in graduate school where he was a member of Mark Akeson’s lab (the UCSC nanopore group; CA, USA). He started as a wet-lab biochemist and transitioned to bioinformatics mid-way through the program. He started working on DNA methylation detection and classification with nanopores from the get-go.
Sayonika Mohanta
Strategic Marketing Manager – Methylation
Oxford Nanopore (Oxford, UK)
Sayonika completed a bioengineering degree at University of California, Los Angeles (CA, USA) and a master’s degree in chemical and biomolecular engineering at Johns Hopkins University (MD, USA). Prior to joining Oxford Nanopore, she worked at a variety of biotechnology companies understanding the current challenges and needs of modern scientists and researchers. Her expertise extends to genomics, where she has adeptly translated complex genomic advancements into compelling and engaging content. In her current role, she employs her deep understanding of methylation and genomics to drive initiatives, working with cross-functional teams and establishing strategic partnerships for the ever-evolving biotech and genomics landscape. She hopes to foster connections and build a community of knowledge sharing between science and industry.
This webinar was recorded on Thursday 24 October 2024
In association with
