Quality control and analysis of gene expression experiments
Gene expression analysis using qPCR quantification of RNA transcripts expressed by cells or viruses plays an important role in the discovery and development of novel biotherapeutics. While widely used, gene expression is a challenging qPCR application that requires researchers to consider various technical and analytical specifications to help ensure results are accurate. In this webinar, John Pfeifer draws on his 30-plus years of field-based expertise to review the importance of quality assurance of qPCR gene expression data and the major quality-control steps needed for reliable results. This talk will provide actionable recommendations to those using gene expression analysis in discovery research and testing for novel therapeutic development.
For Research Use Only-Not for Diagnostic Use
What will you learn?
- Review the best concepts of real-time PCR and gene expression analysis
- Understand the challenges of qPCR gene expression analysis and the importance of proper quality-control steps
- Learn about the three major quality-control steps for quality assurance of qPCR gene expression data
Who may this interest?
- Researchers and scientists using qPCR to perform gene expression analysis
- Scientists that monitor gene expression in bioanalysis workflows for the development of biologics such as cell and gene therapies, monoclonal antibodies (mAbs), or vaccines in research, translational research, preclinical and clinical studies
- Scientists measuring transgenes expression, cell engineering, gene editing, antigen IDS, biodistribution, PK-PD, microbial testing, safety and efficacy testing, in-process testing
Panelists
John Pfeifer, PhD
Senior Real-Time PCR Field Applications Scientist
Thermo Fisher Scientific
As a senior qPCR Field Application Scientist (FAS) with over 30 years of leading industry experience, John Pfeifer partners with researchers to help optimize qPCR workflows via training, troubleshooting and consulting services. Having worked as a qPCR FAS since the technology’s inception, Pfeifer is an expert at guiding customers through the complexities of qPCR to improve lab efficiencies. Pfeifer served as a Postdoctoral Research Fellow at Harvard University (MA, USA) and a Research Associate at Baylor College of Medicine (TX, USA). Pfeifer holds a BSc in genetics from Cornell University (NY, USA) and a PhD in molecular virology from the University of Alabama in Birmingham (AL, USA).
Emily Payne, PhD
Global Market Development Manager
Thermo Fisher Scientific
Emily Payne is a Global Market Development Manager for the qPCR platform at Thermo Fisher Scientific (MA, USA). Emily has a BA in Journalism & Mass Communication from New York University (NY, USA), a BS in Microbiology from the University of Texas at Austin (TX, USA) and a PhD in Experimental Pathology from Duke University (NC, USA).
This webinar was recorded on July 31, 2023.
In association with
